The tissue culture protocol standardized was used for mass multiplication of high yielding disease free (YLD resistant) arecanut palms. Inflorescence explants from disease free palms of age 40 years in farmers’ gardens from hot spot areas in Sullia regions of Karnataka were sampled. Large number of plants was obtained in vitro. Direct somatic embryos from inflorescence rachilla was also obtained, which is a first report. The incidence of YLD had devastated the arecanut growing tracts and only by replanting with resistant planting material the gardens could be rejuvenated. In this context the developed tissue culture protocol has immense field application. The successful development of arecanut tissue culture protocol has opened up new hope in resistance breeding as even with limited number of resistant genotypes, large number of planting material could be produced and distributed among farmers. Besides, large number of newly released dwarf-hybrids can also be produced and distributed among farmers.